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As for the libertarians at places like Reason, they would do well to reflect on what exactly their libertarianism entails. Freedom of speech or the right to choose how and where to be educated? If students are mature enough to choose universities that subject them to religious or modern hate-speech style restrictions or none at all, then libertarians should cheer them on. If students are not mature enough, then libertarians should agree that university codes are not objectionable. We can then argue about what those codes should be, but my main argument stands unrefuted.

To elucidate the role of amh and foxl2 in sex differentiation of the teleost fish Schizothorax kozlovi, the full-length cDNAs were cloned from the mature testis and ovary by rapid amplification of cDNA ends (RACE), and their relative mRNA expression levels were determined by quantitative real-time polymerase chain reaction among tissues and temperature groups. The complete amh and foxl2 cDNAs of S. kozlovi were 2060 bp and 1750 bp, which encoded 568 and 306 amino acids, respectively. The amh were expressed only in gonads, while foxl2 was expressed in the gills, brain and gonads, both exhibiting relatively high tissue specificity. The amh exhibited sex-specific expression pattern in the gonads. No sex differences in the foxl2 expression were observed in the brain and gonads, but significant sex differences were found in the gills. No significant differences were found in the foxl2 expression, from the larval to the juvenile stage, and also between different temperature groups. However, significant differences were found in the expression levels of amh from the larval (12-63 days posthatching (dph)) to the juvenile stage (190 dph), and also among the 18C and 10C groups at 31 dph. This result suggested that amh plays an important role in male sex differentiation of S. kozlovi during the early developmental stage, but no similar effect was observed in foxl2.

Some clinically observable properties of mature gametocytes if gametocytes unaffected by acquired immunity. Immature and mature gametocytes unaffected by host acquired immunity response. The color code used in each panel is the same as for Figure 3. (A) Plot of maximum blood density of mature gametocytes, MaxMG, versus maximum density of intracellular asexual forms, MaxAsx for simulated P. falciparum infections. The simulations were binned by the logarithm of MaxAsx, and horizontal bars show the width of each bin. The logarithmic average of MaxMG are computed for each bin for each of the four types of models color coded. Only simulations that produced mature gametocytes are included, and results are only shown for bins that contained 10 or more simulations. (B) Same as (A), except for simulated P. vivax infections. (C) Plot of ratio of duration of mature gametocytes in the host DurMG to duration of asexual forms DurAsx versus DurAsx for simulated P. falciparum infections. The simulations were binned by the logarithm of DurAsx, and horizontal bars show the width of each bin. The logarithmic average of DurMG/DurAsx are computed for each bin for each of the four types of models color coded. Only simulations that produced mature gametocytes are included, and results are only shown for bins that contained 10 or more simulations. (D) Same as (C), except for simulated P. vivax infections.

The plots in Figure 5 show the effects of these two modalities of host acquired immunity against gametocytes on the production of the mature gametocytes for the simulated P. falciparum infections. (For all the simulated infections considered for these plots, the model innate response is also active.) The fraction of simulations making mature gametocytes, FMG and the mature gametocyte parasite-days, PDMG are shown as functions of two quantities, asexual parasite days PDAsx, and ISGcy. If antibodies acting directly on mature gametocytes are present, they should not affect whether or not an infection generates mature forms, and indeed for this immune modality FMG essentially depends only on PDAsx; see panel 5A. (Remember that the value shown for each tile in panels of Figure 5 is an ensemble average of results of several simulations.) However, if the antibody attacks immature gametocytes instead, then the larger ISGcy is, the smaller FMG. In fact, production of the transmissible forms of P. falciparum is then almost completely suppressed in a large part of the model parameter space even for the largest values of PDAsx; see panel 5B. In addition, for a given PDAsx and ISGcy, PDMG is suppressed more, on average, if antibodies act against the immature gametocytes rather than the mature forms; see panels 5D and 5E. In Additional file 2 there is an explanation of why this might be the case using simplified version of the acquired immunity models.

Effects of gametocyte-specific antibodies in simulated infection on gametocyte duration. Results are from simulated infections in which the host cleared all parasites within three years from primary release of merozoites, and which also made mature gametocytes. The ratio of the duration of mature gametocytes, DurMG to the duration of the asexual forms, DurAsx was binned by DurAsx, and also by a measure of the ability of the antibody response to clear their target, ISGcy. The horizontal length of an individual block shows the bin size in DurAsx, and the vertical extent of a block show the bin size in ISGcy. Results are only shown for bins that contained 10 or more simulations. (A) DurMG/DurAsx for simulated P. falciparum infections in which antibodies attacked mature gametocytes and not immature forms. (B) DurMG/DurAsx for simulated P. falciparum infections in which antibodies attacked immature gametocytes and not mature forms. (C) Same as (A), except for simulated P. vivax infections. (D) Same as (B), except for simulated P. vivax infections. (E) Color code used for DurMG/DurAsx.

Effects of standard deviation of development time of intracellular asexual forms on gametocyte levels. Results are from simulated infections in which the host cleared all parasites within three years from primary release of merozoites, and which also made mature gametocytes. Gametocytes are assumed to be invisible to acquired immunity. The ratio of the parasite days of mature gametocytes to parasite days of asexual forms, PMG/PDAsx, was binned by PDAsx and by the standard deviation of development time of intracellular asexual forms, σAsx. The horizontal length of an individual block shows the bin size in PDAsx, and the vertical extent of a block show the bin size in σAsx. Results are only shown for bins that contained 10 or more simulations. (A) PDMG/PDAsx for simulated P. falciparum infections in which gametocytes are unaffected by host immune responses. (B) Same as (A), except for simulated P. vivax infections. (C) PDMG/PDAsx for simulated P. falciparum infections in which model innate response cleared mature and immature gametocytes at the same rate that it clears asexual forms. (D) Same as (C), except for simulated P. vivax infections. (E) Color code used for PDMG/PDAsx.

Alphestes afer is frequently found in reefs, near coasts or islands. The species is characterized by its robust, small, and laterally compressed body, brown colored with orange little dots, and dark-brown spots, with small and hard scales, rarely reaching a total length of 30 cm (Craig et al. 2006). Alphestes afer is a diachronic hermaphrodite species, changing sex in a protogynic mode, in two different ways: either immature females transform into primary males, or females at mature, spent, or resting stages transform into secondary males (Marques and Ferreira 2011). 041b061a72